University of Wisconsin–Madison

     Welcome to the Pfleger Lab‽

 

We are  multi-disciplined team of researchers focused on using metabolic engineering techniques to produce value-added fuels and chemicals.  With backgrounds ranging from chemical engineering to molecular biology, we are well equipped to create a variety of solutions to any research problem.  We are currently working on a variety of projects in different organisms, including Escherichia coli and Synechococcus sp. 7002.  Led by Professor Brian Pfleger, we hope to solve relevant societal problems using our expertise in biology.

Recent News

Selected Publications

  • Korosh TC, Markley AL, Clark RL, McGinley LL, McMahon KD, Pfleger BF. Engineering Photosynthetic Production of L-lysine. Metabolic Engineering (2017) Nov;44:273-283 DOI: 1016/j.ymben.2017.10.010
  • Rand JM, Pisithkul T, Clark RL, Thiede JM, Mehrer CR, Agnew DA, Campbell CE, Markley AL, Price MN, Ray J, Wetmore KM, Suh Y, Arkin AP, Deutschbauer AM, Amador Noguez D, Pfleger BF. A metabolic pathway for catabolizing levulinic acid in bacteria. Nature Microbiology. Dec;2(12):1624-1634. (2017 DOI: doi:10.1038/s41564-017-0028-z
  • Grisewood MJ, Hernandez Lozada NJ, Thoden J, Gifford N, Mendez-Perez D, Schoenberger HA, Allan MF, Floy M, Lai RY, Holden HM, Pfleger BF, Maranas CD. Computational redesign of acyl-ACP thioesterase with improved selectivity towards medium-chain fatty acids. ACS Catalysis. 7, 3837–3849 (2017) DOI: 1021/acscatal.7b00408
  • Copeland MF, Politz MP, Johnson CB, Markley AL, Pfleger BF. A transcription activator-like effector induction system mediated by proteolytic degradation. Nature Chemical Biology. Apr;12(4):254-60 (2016). DOI: 10.1038/nchembio.2021