Publications

Copeland MF, Politz MP, Johnson CB, Markley AL, Pfleger BF. A transcription activator-like effector induction system mediated by proteolytic degradation. Nature Chemical Biology (Accepted)

Jones CM, Hernandez-Loazada N, Pfleger BF. Efflux Systems in Bacteria and their Metabolic Engineering Applications. Applied Microbiology and Biotechnology. 99(22):9381-93 (2015)

Zess EK, Begemann MB, Pfleger BF. Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. Biotechnology & Bioengineering (2015, Epub online ahead of press)

Luterbacher JS, Alonso DM, Rand JM, Questell-Santiago YM, Yeap JH, Pfleger BF, Dumesic JA. Solvent-enabled nonenzymatic sugar production from biomass for chemical and biological upgrading. ChemSusChem Apr 24;8(8):1317-22. (2015)

Julleson D, David F, Pfleger BF, Nielsen J Impact of Synthetic Biology and Metabolic Engineering on Industrial Production of Fine Chemicals. Biotechnology Advances (Epub online ahead of press)

Pfleger BF, Gossing M, Nielsen J. Metabolic engineering strategies for microbial synthesis of oleochemicals. Metabolic Engineering. May;29:1-11 (2015).

Cameron JC, Gordon GC, Pfleger BF. Genetic and genomic analysis of RNases in model cyanobacteria. Photosynthesis Research. Oct;126(1):171-83. (2015)

Markley AL, Begemann MB, Gordon GC, Pfleger BF. A synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. PCC 7002. ACS Synthetic Biology. May 15;4(5):595-603. (2015)

Mendez-Perez D, Herman NA, Pfleger BF. A desaturase gene involved in formation of 1,14 nonadecadiene produced by Synechococcus sp. Strain PCC 7002. Applied and Environmental Engineering. 80 (19), 6073-6079. (2014)

Politz MP, Copeland MF, Pfleger BF. Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes. Current Opinion in Biotechnology. Mar 12;29C:46-54. (2014)

Clark RL, Cameron JC, Root TW, Pfleger BF. Insights into the Industrial Growth of Cyanobacteria from a Model of the Carbon-Concentrating Mechanism. AICHE Journal. 60(4):1269–1277. (2014).

Luterbacher JS, Rand JM, Alonso DM, Youngquist JT, Pfleger BF, Dumesic JA. Non-enzymatic sugar production from biomass using biomass-derived γ-valerolactone. Science. Jan 17;343(6168):277-80. (2014).

Youngquist JT, Schumacher MH, Rose JP, Raines TC, Politz MC, Copeland MF,  Pfleger BF. Production of medium chain length fatty alcohols from glucose in Escherichia coli. Metabolic Engineering. Nov;20:177-86. (2013).

Begemann MB, Zess EK, Walters EM, Schmitt EF, Markley AL, Pfleger BF. An Organic Acid Based Counter Selection System for Cyanobacteria. PLoS One. Oct 1;8(10):e76594. (2013).

Youngquist JT, Rose JP, Pfleger BF. Free fatty acid production in Escherichia coli under phosphate-limited conditions. Applied Microbiology and Biotechnology. Jun;97(11):5149-59. (2013).

Lennen RM, Pfleger BF. Microbial production of fatty acid derived fuels and chemicals. Current Opinion in Biotechnology Dec;24(6):1044-53. (2013).

Agnew DE, Pfleger BF. Synthetic biology strategies for synthesizing polyhydroxyalkanoates from unrelated carbon sources Chemical Engineering Science. November 15; 103:58-67 (2013).

Lennen RM, Pfleger BF. Engineering unsaturated fatty acid membrane content in free fatty acid exporting Escherichia coli. PLoS One. 8(1):e54031 (2013)

Politz MC, Copeland MF, Pfleger BF. Artificial repressors for controlling gene expression in bacteria. Chemical Communications May 14;49(39):4325-7 (2013).

Lennen RM, Kruziki MA, Politz M, Pfleger BF. Transporters involved in maintaining cell fitness in free fatty acid exporting Escherichia coli. Journal of Bacteriology Jan;195(1):135-44 (2013).

Lennen RM, Pfleger BF. Engineering free fatty acid production in Escherichia coli. Trends in Biotechnology Dec;30(12):659-67 (2012).

Agnew DA, Stevermer AK, Youngquist JT, Pfleger BF. Engineering Escherichia coli for production of C12-C14 polyhydroxyalkanoate from glucose. Metabolic Engineering 14(6):705-713 (2012).

Mendez-Perez D, Gunasekaran S, Orler VJ, Pfleger BF. A translation-coupling DNA cassette for monitoring protein translation in bacteria. Metabolic Engineering Jul;14(4):298-305 (2012).

Youngquist JT, Lennen RM, Ranatunga DR, Bothfeld WH, Marner II WD, Pfleger BF. Kinetic modeling of free fatty acid production in Escherichia coli based on continuous cultivation of a plasmid free strain. Biotechnology & Bioengineering. Jun;109(6):1518-27 (2012).

Graham J, Graham L, Zulkifly S, Hoover SW, Pfleger BF, Yoshitani, J. Freshwater Diatoms as a Source of Lipids for Biofuels. Journal of Industrial Microbiology and Biotechnology. Mar;39(3):419-28 (2012).

Hoover SW, Youngquist JT, Angart PA, Withers ST, Lennen RM, Pfleger BF. Isolation of improved free fatty acid overproducing strains of Escherichia coli via Nile red based high-throughput screening. Environmental Progress & Sustainable Energy. 31(1):17–23 (2012).

Agnew DE, Pfleger BF. (2011) Optimization of synthetic operons using libraries of post-transcriptional regulatory elements. In Methods in Molecular Biology, Strain Engineering: Methods and Protocols (Williams, J. Editor). 765:99-111. (2011).

Lennen RM, Kruziki MA, Kumar K, Zinkel RA, Burnum KE, Lipton MS, Hoover SW, Ranatunga DR, Wittkopp TM, Marner II WD, Pfleger BF. Membrane stresses induced by endogenous free fatty acid overproduction in Escherichia coli. Applied and Environmental Microbiology. Nov;77(22):8114-28 (2011).

Mendez-Perez D, Begemann MB, Pfleger BF. A gene encoding a modular synthase is involved in α-olefin biosynthesis in Synechococcus sp. PCC7002. Applied and Environmental Microbiology. Jun;77(12):4264-7 (2011).

Hoover SW, Marner II WD, Brownson AK, Lennen RM, Wittkopp TM, Yoshitani J, Zulkifly S, Graham LE, Chaston SD, McMahon KD, Pfleger BF. Bacterial production of free fatty acids from freshwater, macroalgal cellulose. Applied Microbiology and Biotechnology. Jul;91(2):435-46 (2011).

Lennen RM, Braden DJ, West RA, Dumesic JA, Pfleger BF. A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes. Biotechnology & Bioengineering. 106:2, 193-202 (2010).

Full Publication List